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The pharmacokinetic parameters assessed after single dose administration on Day 1 were: maximum concentration ( C max ), time to C max ( t max ), terminal elimination rate constant ( z , calculated using log-linear regression from at least three points), area under the concentrationtime curve (AUC) until time t (AUC 0t , calculated using the trapezoidal method), AUC extrapolated to infinity (AUC 0 , calculated as AUC 0t + C t / z , where C t is the last measurable drug concentration), proportion of residual AUC extrapolated to infinity (AUC extra , calculated as 100 [ C t / z ]/AUC 0 ), AUC from administration to 12 h on Day 1 (AUC 012 , calculated using the trapezoidal method), t (calculated as ln2/ z ), volume of distribution [ V z , calculated as dose/(AUC 0 z )] and total body clearance (CL t , calculated as dose/AUC 0 ) of NAC in plasma, and total amount excreted until time t (Ae 0t ), fraction excreted until time t (Fe 0t ) and renal clearance (CL r , Ae 0t /AUC 0 ) of NAC in urine

Protection of proteins from oxidative stress: a new illusion or a novel strategy

Giacconi R, Muti E, Malavolta M, Cipriano C, Costarelli L, Bernardini G, et al

[DOI] [PMC free article] [PubMed] [Google Scholar] 79.Wu X, Wan Q, Wang J, Hou P, Zhang Q, Wang Q, et al

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BLI binding assays were performed in BLI buffer (20 mM TrisHCl, pH 8.0, 150 mM NaCl, 15 mM MgCl 2 , 0.02% LMNG, and 0.1% BSA) and assay mixtures were prepared in black 96-well plates with wells filled to 200 L