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Importantly, the sgRNAs used did not generate off-target mutations across the genome, as confirmed by whole-genome sequencing (Supplementary Fig

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Substrate and coupling reagents were either prepared fresh or thawed from small aliquots stored at 80 C for a maximum of two freeze-thaw cycles

Pastore A, Noce A, Di Giovamberardino G, De Stefano A, Calla C, Zenobi R,

Morbidity and mortality at 3 months The proportion of patients attaining a positive outcome was comparable between the intervention and control groups, with 73.68% (14 patients) and 76.19% (16 patients), respectively (95% CI 24.3 to 29.4