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Elispot assay detection of cytokine secretion in multiple sclerosis patients treated with interferon-beta1a or glatiramer acetate compared with untreated patients

doi: 10.3390/pharmaceutics13010010 24 JiaJZhuFMaXCaoZWLiYXChenYZ

Western blot Total protein extracts from macrophages (1 10 6 cells) were prepared using RIPA lysis buffer containing protease and phosphatase inhibitors (Thermo Fisher Scientific)