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For example, MNX mice have been created using C57BL6/J and C3H/HeN mice to explore obesity and metabolic syndrome ( 1.3.1 Cytoplasmic hybrid (cybrid) cell lines First derived in 1975 by Wallace, Bunn, and Eisenstadt, cytoplasmic hybrid (cybrid) cell lines are generated by the fusing of nucleated cells that have had their endogenous mtDNA depleted via exposure to ethidium bromide with enucleated cells (cytoplasts), which harbor the mtDNA signature of interest (Figure 3A

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