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The sample was analyzed via UV-Vis, then the temperature was raised of 4 K at a time stirring the solution for 1 min before leaving the system to equilibrate for another 15 min and repeating the acquisition every 5 min until two consecutive spectra are superimposable between 288 and 298 K, then 2 K per step until 327 K

The reaction was stopped by adding 100 l of stop solution into each well

second, the VTA models may approximate the real volume of tissue activated but not fully represent reality, and the estimation of the VTA did not involve the impact of stimulating frequency

Statistical analysis All experiments contained at least three independent replicates

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Again, it may take a few weeks before your vitamin B12 levels rise and symptoms start to improve