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Human monocytic leukemia THP-1 cells were maintained in RPMI 1640 media supplemented with 2 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10% FBS, 5% penicillinstreptomycin (Gibco, Thermo Fisher Scientific), and amphotericin B (Gibco, Thermo Fisher Scientific)

So there is a dose finding effect and everyone is personal

5.6 Musculoskeletal Pain In post-marketing experience, severe and occasionally incapacitating bone, joint, and/or muscle pain have been infrequently reported in patients taking bisphosphonates, including Reclast

D., Viscogliosi, E

Glutathione Assay and Melasma Examination Blood samples were taken through a cubital vein, a glutathione examination kit using the QuantiChrom TM Glutathione Assay Kit (Elisa Method)