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Use the detailed reconstitution and dosage guide to verify your concentration calculations and syringe measurement approach

pDNA concentration and purity were measured on a NanoDrop One (Thermo Fisher, UK), and then linearized using MluI for 3 h at 37 C, followed by heat inactivation at 80 C for 20 min

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At the protein level, circadian oscillation of clockwork negative factors such as PERs and CRYs were expected to be in anti-phase with BMAL1, and to be more highly expressed during the dark period relative to the night based on studies on mouse SCN (36)