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We then used a Cryostat Microtome CM3050S (Leica, Germany) to section the muscle sample, the tissue sections were stored at 80 C for later Hematoxylin and Eosin stain (H&E stain), Gmri trichrome stain, and nicotinamide adenine dinucleotide dehydrogenasetetrazolium reductase (NADH-TR) stain as described previously 28