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Parallel Reaction Monitoring of Putative Adducts Samples from wild-type, Xpa -/- , and Xpc -/- liver DNA were prepared for LC-MS 2 analysis of putative DNA adducts detected in the screening assay using an Orbitrap Lumos instrument (ThermoFisher) coupled to a UHPLC system (Ultimate 3000 RSLCnano UHPLC, ThermoFisher) using positive NSI with the source temperature of 300 C and the spray voltage set to static at 2200 V

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Implementing specific practices and making lifestyle adjustments post-treatment significantly contributes to prolonging the positive effects of the procedure

Cell viability, apoptosis, and membrane integrity were assessed

10.1021/jacs.5b04350 J

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