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Assuming that the complete purication of ergothioneine would further reduce the Amberlite IRC120 H column yield to 1% or less, as little as 3.64 kg of this biomass waste would be required to provide a milligram of high-purity ergothioneine

How they are removed - which exact treatments are needed - is very individualized

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Additionally, the mix contains factors that enhance PCR amplification efficiency and promote balanced amplification across a wide range of GC content (30-70%), ensuring good linearity in quantitative PCR over a broad quantification range

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