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Single-cell suspensions were then loaded onto microfluidic devices, and single-cell RNA sequencing (scRNA-seq) libraries were constructed according to the Singleron GEXSCOPE protocol via the GEXSCOPE Single-Cell RNA Library Kit (Singleron), which included cell disruption, mRNA trapping, cell labeling (with barcodes) and mRNA labeling (with unique molecular identifier (UMI)), reverse transcription of mRNA into complementary DNA (cDNA) and amplification, cDNA fragmentation, and finally cDNA fragment cloning

Biochem Biophys Res Commun 363:921925 Wu P, Dong J, Cheng N, Yang R, Han Y, Han Y (2019) Inflammatory cytokines expression in Wilsons disease

Access this article Similar content being viewed by others References YS Ilhan N Bulbuller C Kirkil R Ozercan D Seckin (2005) ArticleTitleThe effect of an angiotensin converting enzyme inhibitor of intestinal wound heaking J Surg Res 128 615 Occurrence Handle15869762 Occurrence Handle1:CAS:528:DC%2BD2MXpsVWks7k%3D P Sikiric S Seiwerth Z Grabarevic M Petek R Rucman B Turkovic et al

This doesn't necessarily mean that all vegetarians need B12 injections, but it is advisable for vegans and vegetarians be mindful of their B12 intake to ensure they are getting sufficient levels of this vital nutrient

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