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It is important to consider caveats in studies on mitochondrial bioenergetics, electron, and proton leak including the penetrance of mitochondrial inhibitors and the challenge of uniformly delivering drugs to all cells within islets

To avoid the osmotic shock, salinity treatment was applied gradually at the rate of 25 and 50 mM NaCl per day for 50 and 100 mM NaCl treatments, respectively for two days to maintain the required concentration

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