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The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (ET1702-09) at 1/1,000 dilution for 1 hour at room temperature

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Simply hold the syringe needle-up, tap the side to encourage the bubble to rise to the top, and then gently push the plunger to expel the air before proceeding with your research application

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Since most people already consume too much salt, its likely best to avoid sole water