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Immunohistochemistry (IHC) IHC analysis was performed with 4-hydroxynonenal (4-HNE), Nitrotyrosine, TUNEL apoptotic staining, BrdU, Ki-67, PCNA antibodies and HIF-1, using methods as previously described 27

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In fact, different products of PCs are generated from oxidative assault to amino acid side chains or backbone cleavage in a protein by hydroxyl radicals 39