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For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)

REF-2383 2/2025

Dietary anthocyanins as nutritional therapy for nonalcoholic fatty liver disease

Research has demonstrated that GA modulates the AMPK/SREBP/ACC and IRs-1/Akt signaling pathways, which are pivotal in cellular metabolism, and it also suppresses liver inflammation by targeting the IKK/i-B/NF-B signaling pathway, a key mediator of inflammatory responses ( Previous studies on the mechanism of action of GA on NAFLD were based on pharmacological methods

Notably, although plant metabolites such as berberine, emodin and curcumin have been shown in vitro to improve NAFLD symptoms by regulating hepatic lipid metabolism and inhibiting hepatocellular inflammation, they are categorized as pan-assay interference compounds (PAINS), which may generate false-positive results in vitro through non-specific mechanisms (Magalhes et al., 2021)

Back Klausen, MK, Jensen, ME, Mller, M, et al (6 Sept 2022) 'Exenatide once weekly for alcohol use disorder investigated in a randomized, placebo-controlled clinical trial', JCI Insight, 7/19, e159863