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Both genes were cloned separately into the pPink-HC expression vector multiple cloning site (MCS) using EcoRI and FseI (NEB)

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Uriu-Adams JY, Keen CL (2005) Copper, oxidative stress, and human health

The primer selection (V5-V6) used in this study allowed us to capture a wide range of bacteria, including Bifidobacteria, which are typically not captured when using other primers and regions such as V3-V4 (55)

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