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After stirring 1 h, the reaction mixture was stirred at room temperature for 16 h

In an embodiment, the coding nucleic acid sequence encodes a protein of interest for Gram-Negative Bacteria

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12928-052-228 CAM: 765398-228052 Formula (Vila) Formula (Vllb)

Examples of chemical bonds include, but are not limited to, those based on carbamates, ethers, esters, amides, imines, amidines, aminotrizines, hydrozone, disulfides, thioethers, thioesters, phosphorothioates, phosphorodithioates, sulfonamides, sulfonates, fulfones, sulfoxides, ureas, thioureas, hydrazide, oxime, triazole, photolabile linkages, C-C bond forming groups such as Diels-Alder cyclo-addition pairs or ring-closing metathesis pairs, and Michael reaction pairs

To a solution of triethyl phosphonoacetate (11.2 g, 50.1 mmol) in THF (60 mL) cooled to 15 C