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This dose represents the optimal balance between efficacy and tolerability for the majority of users, providing robust NNMT inhibition without excessive stimulation

Yamaura K, Ogawa K, Yonekawa T, Nakamura T, Yano S, Ueno K

Female 5xFAD mice that express human APOE4 (E4FAD) exhibit increased anxiety-like behavior relative to E3FAD, and ovariectomy (OVX) in E3FAD mice increases anxiety to a similar level to intact and OVXed E4FAD mice, suggesting a protective role of ovarian hormones against anxiety-like phenotypes in E3FAD but not E4FAD mice (Taxier et al., 2022)

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Erratum in Nat

Cells were washed in PBS, resuspended in Thiazole Orange (BD Retic-Count, BD Bioscience) containing 4 M Hoechst 33342 (Invitrogen) for parasite RNA and DNA detection respectively ( 2 , 5 mM glucose, 32 mM HEPES pH7.4, 5 mM KCl, 1 mM MgSO4, 125 mM NaCl), resuspended in 0.3 M of intracellular calcium dye x-Rhod-1 AM (Invitrogen) and incubated in the dark at 37C for 30 min