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Samples were allowed to cool, and 1.375 ml purified water with a resistivity 18.2 M cm from a Milli-Q system (Merck Millipore) was added to make up to a final volume of 1.5 ml

Sunlight effects on the osmotrophic uptake of DMSP-sulfur and leucine by polar phytoplankton

After 2 weeks, animals with blood glucose values above 180 mg/dL were selected to perform the tests described below

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Conditioned medium experiment

The M9 was aspirated from the tube and replaced with 1mL fresh M9 and spun again