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Department of Experimental Medicine, Section of General Pathology, Via LB Alberti 2, 16132 Genoa, Italy Read Full Article here Yang, L., & Zhang, H

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a,b , Representation of the stationary points calculated with ONIOM(B97X-D/6-311 + G(2d,p):ff14SB,gaff2,TIP3P) G(d):ff14SB,gaff2,TIP3P) for the Pt GSTF8-catalysed deprotonation-protonation reaction: flavan-3-on-4-ol ( 7 ), transition state for deprotonation ( 7-TS ), deprotonation product ( 8-enolate ) and cyanidin ( 8-B4 ) bound to the wild-type enzyme ( a ) and the C13S variant ( b )

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For NMR analysis, polar fractions were resuspended in 630 l of PBS (pH 7.4), adding 70 l of 2 H 2 O solution (containing 1 mM sodium 3-trimethylsilyl [2,2,3,3-2H4]propionate (TSP) as a chemical shift reference for 1 H spectra) to provide a field frequency lock, reaching 700 l of total volume

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