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In the next step, samples were diluted with 100 mM ABC, then reduced with 5 mM TCEP (in 50 mM ABC) for 30 min at 37 C, re-alkylated with 10 mM d5-NEM, diluted with four volumes of 25 mM ABC and digested overnight with trypsin at 37 C, (shaking at 550 rpm

The mutagenicity of trichloroethylene should also be validated in additional cohorts

As was the case for research done with animal subjects, human trials pointed to epitalon benefits desirable for individuals of all ages

Lasso et al., 2022