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mRNA gene expression in the intestinal mucosa To quantify relative mRNA expression levels of interferon (IFN)-, tumor necrosis factor (TNF)-, interleukin (IL)-6 mRNA was extracted from intestinal mucosa samples (ileum and cecum) using the miRNeasy mini kit (QIAGEN)

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Future model development should integrate innovative technologies to enhance the authenticity of pathological features, address the shortcomings of existing systems, and facilitate the clinical translation of GA research

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Research background matters because peptide handling, sequence stability, and route-specific formulation all affect interpretation