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Lin et al., 2014)

DKD patients exhibit significant structural dysbiosis of gut microbiota, characterized by decreased abundance of beneficial bacteria producing short-chain fatty acids (SCFAs) and increased abundance of harmful bacteria generating uremic toxin precursors (12)

The insert of construct pAGB676 (contains hrcL to hrcT and hrcC with a deletion) was subsequently assembled with the insert of pAGB197 (contains hrpB1 to hrpB4 ) in the Bpi I sites of vector pICH41331, resulting in the level 0 construct pAGB680

Finally, the purity of the fractions was confirmed by Western blot analysis using cytoplasmic marker protein GAPDH and nuclear marker protein Histone H3(HH3)

Pharmacol Res 146:104332104346