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Estimation of APAP levels in serum using high-performance liquid chromatography (HPLC) To confirm obtaining toxic levels, an estimation of the APAP level in serum was performed

They electrically stimulated the intralaminar thalamic area of rabbits to induce sleep, collected the cerebral venous blood via extracorporeal dialysis, and fractionated the dialysate hunting for the substance responsible

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After an additional 4 days ( t = 96 h), cells were collected by trypsinization, pelleted, snap-frozen and stored at 80 C