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To further analyze whether apoptosis would coexist with necrosis in APAP-induced hepatotoxicity, we detected APAP-induced apoptosis by TUNEL staining and investigated the possible role of Bcl-2 family proteins and caspase-3 under this pathophysiological condition

After incubation, the cells were harvested and centrifuged at 300 g for 10 min

Whether this phenotype reflects hyper-acidification or elevated lysosomal pH may depend on the experimental context 164,167

With the former, the penis will usually end up half its present length because the added girth will cause the penis to retract further into the fat pad

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