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Treatment with 200 M H 2 O 2 successfully induced a robust senescence model in HUVECs ( 2 O 2 -only treatment group (Figure 2G), and the concentration of 0.05 uM of BS had the least effect on the viability of the cells, and -gal staining further confirmed that 0.05 M of BS significantly attenuated hydrogen peroxide-induced HUVEC senescence (Figures 2F,H)

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Protocols are entirely community-developed, with doses extrapolated from cell culture bioactivity data and molecular weight calculations

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GAPDH served as the internal control