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Description

S.GaviglioE

Mrx-1 operates via a monothiol reaction mechanism in the de-mycothiolation reaction analogous to most glutaredoxins that are involved in de-glutathionylation

Kostaropoulos I, Mantzari A, Papodopoulos AI

311 Wilhelm et al

Before each round, the library or each ssDNA pool was heated at 90 C for 10 minutes, immediately cooled, and kept at 4 C for 15 min, then briefly incubated (58 min) at room temperature before their application in the binding reaction