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The brain was quickly removed, and coronal slices (250 m thick) containing the ventral striatum were cut using a vibrating tissue slicer (Campden Instruments) in oxygenated (95% O 2 / 5% CO 2 ) ice-cold modified artificial CSF (ACSF), containing (in mM): 105 sucrose, 65 NaCl, 25 NaHCO3, 2.5 KCl, 1.25 NaH 2 PO 4 , 7 MgCl 2 , 0.5 CaCl 2 , 25 glucose, 1.7 L(+)-ascorbic acid

However, alcohol can be detected in urine for a longer period after consumption, making it less accurate for current BAC levels

Pest Manag Sci 65(5):460466

E., Muoz Forti, K., Weisman, G