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At 1.5 h of incubation, 50 l supernatant was removed to a new 96-well plate for glycerol analysis and a fresh 50 l stimulation buffer was added before placing the plate back in the incubator

Directions: Take (2) Glow Nubian pills and (1) Vitamin C pill TWICE daily

subtilis , the Mn 2+ and Fe 2+ quotas are often in the range of 500 M averaged over the cell, with perhaps 1-2% of these ions in a rapidly exchangeable, labile pool (~10 4 atoms per cell) (Helmann, 2014)