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Heat inactivated serum was included as negative control

doi: 10.1136/gutjnl-2021-326789 13 DeCandiaA

Furthermore, none of the compounds showed hERG inhibition or hepatotoxicity, except for compound 4, which was predicted to be potentially hepatotoxic

The other two detected cysteines, C87 and C252, were not methylated, while cysteines forming the active site, C157 and C207, were not detected in our assay, even with various proteinase digestion methods