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Treg cells are a major factor in creating an immunosuppressive tumor microenvironment

After TMB substrate solution is added, only those wells that contain Pig IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

doi: 10.1080/00480169.2007.36763 68 PoldersMHoltershinkenMMeyerURehageJFlachowskyG

Quantitation was performed using separate weighted (1/ x 2 for both analytes) linear least squares regression analyses generated from calibration standards