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0.4 mm distance) were prepared using a Leica CM1520 cyrostat (Leica Biosystems, Wetzlar, Germany) at a constant temperature of -15 C, and stained with cresyl violet (Merck Millipore, Darmstadt, Germany, #105235) according to manufacturer's instructions

They are important signaling intercessors, playing roles in tumor growth, TME remodeling, metastasis, angiogenesis, as well as treatment resistance [260, 261]