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Immunohistochemistry For immunohistochemical analysis of MBP and 4,6-diamidino-2-phenylindole (DAPI), tissue was fixed on slides with 4% paraformaldehyde for 20 min at room temperature, followed by three washes of 1 PBS

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Lira was found in PA-induced Kupffer cells to reduce HF diet-induced hepatic steatosis by inhibiting NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome activation [62]

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