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Description

CFTR is thought to be involved in sperm capacitation and osmoregulation via interactions with ion and water channels

Number of days each animal to be housed - 1 months Result of Atomic Absorption Spectroscopy (AAS) study As per the study design, blood samples were collected from the study animals at predetermined time interval

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Crypts ( N = 500) per well were seeded in 50 l Matrigel (BD, 356231) on a pre-warmed 24-well plate and allowed to solidify for 10 min at 37 C, after which 500 l IntestiCult Growth Medium supplemented with 100U/ml penicillin and 100 g/ml streptomycin (Biochrome, 0257F) was added

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Statements Author contributions RA: Supervision, Investigation, Writing review & editing, Conceptualization, Software, Methodology, Resources, Funding acquisition, Project administration, Validation, Formal analysis, Writing original draft, Visualization, Data curation