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Natural Medicines 16 (9), 700713

429 The pathogenesis of POLG -related disorders has been discussed previously, but its important to note that different mutant genes involved in mtDNA replication may lead to distinct patterns of mtDNA deletions, varying in size and location

Lentiviruses were generated in 293 T cells using polyethylenimine (PEI) and a transient three plasmid transfection protocol: the above-mentioned pHAGE-DsRED vector with the insert, psPAX2 (a gift from Didier Trono (Addgene plasmid # 12260)), and pCMV-VSV-G (a gift from Bob Weinberg (Addgene plasmid # 8454))

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