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Free heme, even at 50100 M concentration, is a toxic molecule due to its ability to generate ROS (Gutteridge and Smith, 1988) catalyzing the oxidation of proteins, the formation of cytotoxic lipid peroxides via lipid peroxidation and damaging DNA (reviewed in Kumar and Bandyopadhyay, 2005)

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SHLP3 decreases gentamicin-induced activation of AKT and AMPK in cochlear cells The mechanism by which SHLP3 exerts cytoprotective effects is largely unknown, but it was hypothesized to function similarly to HN