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Abundance represents the metabolite level difference between conditioned and virgin media (n = 3 technical replicates from one experiment)

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These structural changes encompass various facets, including epithelial damage, goblet and mucus cell hyperplasia leading to mucus overproduction, subepithelial fibrosis contributing to heightened airway stiffness, hypertrophy of airway smooth muscle (ASM) resulting in increased airway constriction, and structural alterations in the airway vasculature ( 2 The impact of obesity on asthma outcome and airway remodeling Numerous studies have been conducted to comprehend the impact of obesity on the clinical outcomes of individuals with asthma, consistently revealing that obesity plays a pivotal role in exacerbating asthma severity and contributes to a spectrum of adverse clinical outcomes (Table 1)