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PVDF membranes after blocking were incubated with primary 6His tag monoclonal antibody (1:1,000 dilution) and GAPDH loading control monoclonal antibody (1:2,500 dilution) at 4 C overnight

In control siRNA-treated cells, MxB protein was mostly associated with nuclear fractions, consistent with previously published results 14

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Receptor for advanced glycation endproducts: a multiligand receptor magnifying cell stress in diverse pathologic settings[J]

This supports balanced hormone levels and reduces the risk of side effects often associated with synthetic hormone therapies