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Lipid droplets can be visualized using Nile red dye, which stains neutral lipids (Fig 4H)

Blots were then probed with the relevant primary antibodies in 5% BSA, 0.1% Tween-20 and 1 PBS or in 5% non-fat skimmed milk powder in 0.1% Tween-20 and 1 PBS at 4 C overnight with gentle motion in a hand-sealed transparent plastic bag