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[DOI] [PubMed] [Google Scholar] 56.Temple R

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Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95

The absence of new OLGs in shadow plaques led the authors to conclude that myelin repair in the human brain does not stem from OPCs, instead originating from surviving OLGs that form new myelin sheaths