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Based on the activities of S586A, S586G, and S586T compared with WT, it is speculated that there are specific requirements regarding the steric hindrance and hydrophobicity of residue 586

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99 Daily doses of at least 50100 g are needed in cases of maternal deficiency, and should improve inadequate levels in the infant without exposing the infant to excessive B12

Use a new sterile needle and syringe for each withdrawal to maintain sterility

Timing considerations: Complete baseline 1-2 weeks before starting peptides allowing clear before/after comparison