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The GSH/GSSG ratio was calculated by dividing the GSH content by the GSSG content

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For real-time PCR, RNA was extracted using RNAStat60 (Tel-Test) and treated with RQ1 DNAse (Promega, no

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Gclc is required for T cell-independent and -dependent B cell immune responses Next, we aimed to understand how the metabolic perturbations caused by B cell-specific Gclc ablation impinge on immune responses and in particular how the impaired mitochondrial metabolism affects the antibody response

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