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The amino acids were accurately mixed on a 0.02 mol/L HCL vortexer, passed through a 0.22 m filter column and the amino acids were determined for each sample in an amino acid analyser (Hitachi, L-8900, Japan)

This solution was prepared in advance, and the entire mixture was incubated in a shaker incubator at 37 C for 24 h, as determined by previous experiments to be the optimal conditions for gene silencing

287,288 Even passive smokers (those exposed to second-hand smoke) have been found to have higher likelihood and severity of menopausal hot flashes compared with non-smokers

[130] [131] [132] [133] Psychological It has also been hypothesized that multiple chemical sensitivity is a psychological disorder

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doi: 10.1016/j.ijbiomac.2010.08.007