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after what, samples were washed twice with 3% BSA in PBS for 2 min each

Janku F, Huang HJ, Fujii T, Shelton DN, Madwani K, Fu S, Tsimberidou AM, Piha-Paul SA, Wheler JJ, Zinner RG, Naing A, Hong DS, Karp DD, Cabrilo G, Kopetz ES , Subbiah V, Luthra R, Kee BK, Eng C, Morris VK, Karlin-Neumann GA, Meric-Bernstam F

Curcumin, a natural NF-B inhibitor, has been studied in the context of neurodegenerative and metabolic diseases, but its therapeutic value remains inconsistent owing to its poor bioavailability and rapid metabolism

Bolouki, A., Zal, F

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Mutations in these regions can disturb GTP binding and lead to decreased sensitivity of GDH to allosteric regulation by GTP, resulting in weakened inhibitory effect and enhanced GDH activity [12]