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By inducing mild oxidative stress, they can activate endogenous defense systems, particularly the Nrf2 pathway, which plays a central role in regulating antioxidant responses

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The RNA and primers were then annealed by incubating as follows: 90 C (1 min), 80 C (1 min), 70 C (1 min), 60 C (1 min), 50 C (1 min), and 40 C (6 min)

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Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by thymoquinone-rich fraction and thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes

Understanding this pathway is not only key to elucidating the origin of SFN but also provides the essential toolkit for its sustainable enhancement through genetic biofortification