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Following successful amplification by monoplex PCR, two distinct pools containing the 21 optimized primer pairs were used for multiplex PCR, maintaining a final primer concentration of 0.1M

: 1.56uM Adenosine cell, tissue lysates, serum, plasma, urine . : Adenosine Standard10X Assay BufferFluorometric ProbeHRPAdenosine Deaminase Purine Nucleoside PhosphorylaseXanthine OxidaseInosine Assay Kit (#MET-5092)Inosine DNA nucleoside tRNA . Inosine microplate-based . : 300 nM Inosine cell, tissue lysates, serum, plasma, urine . : Inosine Standard10X Assay BufferFluorometric ProbeHRPPurine Nucleoside PhosphorylaseXanthine OxidaseGuanosine Assay Kit (#MET-5149)Guanosine GMP, cGMP, GDP, GTP

At the initial baseline visit, serial venous sampling performed after oral d 3 -l-carnitine challenge revealed rapid increases in plasma concentrations of d 3 -l-carnitine, and subsequent increases in d 3 -BB and d 3 -TMAO following a lag phase (Figure 1, AC, filled circles)

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[DOI] [PubMed] [Google Scholar] 296.Makrecka M, Kuka J, Volska K, Antone U, Sevostjanovs E, Cirule H, et al

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