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Nov 7 2025;doi:10.1056/NEJMoa2513032

But avoiding these mistakes is equally important

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Reaction was stopped by adding 2N H 2 SO 4 and absorbance was recorded at 450 nm

Some protocols involve monthly 10-day cycles, while others use longer 20-day cycles every 3-6 months

the concentration is calculated via integration of the peak area/height by the external standard method for the reduced fraction and the internal standard method for the total GSH fraction