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Description

Destruction of the methylated, parental DNA plasmid by DpnI digestion followed by E.coli cells transformation allowed the introduction of the desired mutations

The resulting data were preprocessed and analyzed with RnBeads 67 , and we selected 1,072 genomic regions (corresponding to 16 core regions, 32 additional regions, and 1024 further regions for the EnrichmentBS assays) from the list of Infinium probes that passed quality control