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To determine if any of the Cys residues are modified by palmitate they were individually mutated to Ser (V5_GSTP1-Cys15Ser, V5_GSTP1-Cys48Ser, V5_GSTP1-Cys102Ser, and V5_GSTP1-Cys170Ser) and expressed transiently in HEK293T cells

Sun Y, He L, Wang T, Hua W, Qin H, Wang J, et al

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Cell Biol Toxicol 16:145153 Ronco C, Cicoira M, McCullough PA (2012) Cardiorenal syndrome type 1: pathophysiological crosstalk leading to combined heart and kidney dysfunction in the setting of acutely decompensated heart failure

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The same trend was measured for the Albu-LiTE constructs, despite that more of the constructs were detected in the medium, possibly due to anti-EGFR nanobody in the LiTE construct binding to EGFR on the cell surface of the HMEC-1 cells (Supplementary Fig