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Western blot analysis Preparations of cell lysates and Western blot cells or tissues were lysed in RIPA buffer (phosphate-buffered saline containing 1% Nonidet P-40, 0.1% sodium dodecyl sulfate [SDS], 1 mmol/L Na3VO4, 4 mm phenylmethylsulfonyl fluoride and 0.05% [w/v] apro-tinin)

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These could include mild issues like injection site reactions as well as nausea and upset stomach

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187 Investigators have therefore hypothesized that aerosols of hyaluronic acid inhaled daily may protect against lung elastic fiber injury